OrangerV1, Main, Exploration, bibRecord, 001113

Structural Characterization of the Thermally Tolerant Pectin Methylesterase Purified from Citrus sinensis Fruit and Its Gene Sequence

Identifieur interne : 001113 ( Main/Exploration ); précédent : 001112; suivant : 001114

Structural Characterization of the Thermally Tolerant Pectin Methylesterase Purified from Citrus sinensis Fruit and Its Gene Sequence

Auteurs : Brett J. Savary [États-Unis] ; Prasanna Vasu [États-Unis, Inde] ; Randall G. Cameron [États-Unis] ; T. Gregory Mccollum [États-Unis] ; Alberto Nunez [États-Unis]

Source :

Journal of agricultural and food chemistry : (Print) [ 0021-8561 ] ; 2013.

RBID : Pascal:14-0041424

Descripteurs français

Pascal (Inist)
- Caractérisation, Tolérance, Pectinesterase, Fruit, Séquence nucléotide, Citrus sinensis, Paroi cellulaire, Jus d'orange, Stabilité thermique, MALDI, Réaction chaîne polymérase, Purification.
Wicri :
- topic : Fruit.

English descriptors

KwdEn :
- Cell wall, Characterization, Citrus sinensis, Fruit, Matrix assisted laser desorption ionization, Nucleotide sequence, Orange juice, Pectinesterase, Polymerase chain reaction, Purification, Thermal stability, Tolerance.

Abstract

Despite the longstanding importance of the thermally tolerant pectin methylesterase (TT-PME) activity in citrus juice processing and product quality, the unequivocal identification of the protein and its corresponding gene has remained elusive. TT-PME was purified from sweet orange [Citrus sinensis (L.) Osbeck] finisher pulp (8.0 mg/1.3 kg tissue) with an improved purification scheme that provided 20-fold increased enzyme yield over previous results. Structural characterization of electrophoretically pure TT-PME by MALDI-TOF MS determined molecular masses of approximately 47900 and 53000 Da for two principal glycoisoforms. De novo sequences generated from tryptic peptides by MALDI-TOF/TOF MS matched multiple anonymous Citrus EST cDNA accessions. The complete tt-pme cDNA (1710 base pair) was cloned from a fruit mRNA library using RT- and RLM-RACE PCR. Citrus TT-PME is a novel isoform that showed higher sequence identity with the multiply glycosylated kiwifruit PME than to previously described Citrus thermally labile PME isoforms.

Affiliations:

Inde, États-Unis

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000028
to stream PascalFrancis, to step Curation: 000972
to stream PascalFrancis, to step Checkpoint: 000034
to stream Main, to step Merge: 001121
to stream Main, to step Curation: 001113

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Structural Characterization of the Thermally Tolerant Pectin Methylesterase Purified from Citrus sinensis Fruit and Its Gene Sequence</title>
<author><name sortKey="Savary, Brett J" sort="Savary, Brett J" uniqKey="Savary B" first="Brett J." last="Savary">Brett J. Savary</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor</s1>
<s2>Pennsylvania 19038</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Pennsylvania 19038</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Arkansas Biosciences Institute, Arkansas State University, P.O. Box 639, State University</s1>
<s2>Arkansas 72467</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Arkansas 72467</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Vasu, Prasanna" sort="Vasu, Prasanna" uniqKey="Vasu P" first="Prasanna" last="Vasu">Prasanna Vasu</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Arkansas Biosciences Institute, Arkansas State University, P.O. Box 639, State University</s1>
<s2>Arkansas 72467</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Arkansas 72467</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Food Safety and Analytical Quality Control Laboratory, Central Food Technological Research Institute, CSIR</s1>
<s2>Mysore 570 020</s2>
<s3>IND</s3>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>Inde</country>
<wicri:noRegion>Mysore 570 020</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cameron, Randall G" sort="Cameron, Randall G" uniqKey="Cameron R" first="Randall G." last="Cameron">Randall G. Cameron</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 2001 South Rock Road</s1>
<s2>Fort Pierce, Florida 34945</s2>
<s3>USA</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Fort Pierce, Florida 34945</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mccollum, T Gregory" sort="Mccollum, T Gregory" uniqKey="Mccollum T" first="T. Gregory" last="Mccollum">T. Gregory Mccollum</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 2001 South Rock Road</s1>
<s2>Fort Pierce, Florida 34945</s2>
<s3>USA</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Fort Pierce, Florida 34945</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nunez, Alberto" sort="Nunez, Alberto" uniqKey="Nunez A" first="Alberto" last="Nunez">Alberto Nunez</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor</s1>
<s2>Pennsylvania 19038</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Pennsylvania 19038</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">14-0041424</idno>
<date when="2013">2013</date>
<idno type="stanalyst">PASCAL 14-0041424 INIST</idno>
<idno type="RBID">Pascal:14-0041424</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000028</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000972</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000034</idno>
<idno type="wicri:doubleKey">0021-8561:2013:Savary B:structural:characterization:of</idno>
<idno type="wicri:Area/Main/Merge">001121</idno>
<idno type="wicri:Area/Main/Curation">001113</idno>
<idno type="wicri:Area/Main/Exploration">001113</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Structural Characterization of the Thermally Tolerant Pectin Methylesterase Purified from Citrus sinensis Fruit and Its Gene Sequence</title>
<author><name sortKey="Savary, Brett J" sort="Savary, Brett J" uniqKey="Savary B" first="Brett J." last="Savary">Brett J. Savary</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor</s1>
<s2>Pennsylvania 19038</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Pennsylvania 19038</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Arkansas Biosciences Institute, Arkansas State University, P.O. Box 639, State University</s1>
<s2>Arkansas 72467</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Arkansas 72467</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Vasu, Prasanna" sort="Vasu, Prasanna" uniqKey="Vasu P" first="Prasanna" last="Vasu">Prasanna Vasu</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Arkansas Biosciences Institute, Arkansas State University, P.O. Box 639, State University</s1>
<s2>Arkansas 72467</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Arkansas 72467</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Food Safety and Analytical Quality Control Laboratory, Central Food Technological Research Institute, CSIR</s1>
<s2>Mysore 570 020</s2>
<s3>IND</s3>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>Inde</country>
<wicri:noRegion>Mysore 570 020</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cameron, Randall G" sort="Cameron, Randall G" uniqKey="Cameron R" first="Randall G." last="Cameron">Randall G. Cameron</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 2001 South Rock Road</s1>
<s2>Fort Pierce, Florida 34945</s2>
<s3>USA</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Fort Pierce, Florida 34945</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mccollum, T Gregory" sort="Mccollum, T Gregory" uniqKey="Mccollum T" first="T. Gregory" last="Mccollum">T. Gregory Mccollum</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 2001 South Rock Road</s1>
<s2>Fort Pierce, Florida 34945</s2>
<s3>USA</s3>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Fort Pierce, Florida 34945</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nunez, Alberto" sort="Nunez, Alberto" uniqKey="Nunez A" first="Alberto" last="Nunez">Alberto Nunez</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor</s1>
<s2>Pennsylvania 19038</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Pennsylvania 19038</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of agricultural and food chemistry : (Print)</title>
<title level="j" type="abbreviated">J. agric. food chem. : (Print)</title>
<idno type="ISSN">0021-8561</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of agricultural and food chemistry : (Print)</title>
<title level="j" type="abbreviated">J. agric. food chem. : (Print)</title>
<idno type="ISSN">0021-8561</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell wall</term>
<term>Characterization</term>
<term>Citrus sinensis</term>
<term>Fruit</term>
<term>Matrix assisted laser desorption ionization</term>
<term>Nucleotide sequence</term>
<term>Orange juice</term>
<term>Pectinesterase</term>
<term>Polymerase chain reaction</term>
<term>Purification</term>
<term>Thermal stability</term>
<term>Tolerance</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Caractérisation</term>
<term>Tolérance</term>
<term>Pectinesterase</term>
<term>Fruit</term>
<term>Séquence nucléotide</term>
<term>Citrus sinensis</term>
<term>Paroi cellulaire</term>
<term>Jus d'orange</term>
<term>Stabilité thermique</term>
<term>MALDI</term>
<term>Réaction chaîne polymérase</term>
<term>Purification</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Fruit</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Despite the longstanding importance of the thermally tolerant pectin methylesterase (TT-PME) activity in citrus juice processing and product quality, the unequivocal identification of the protein and its corresponding gene has remained elusive. TT-PME was purified from sweet orange [Citrus sinensis (L.) Osbeck] finisher pulp (8.0 mg/1.3 kg tissue) with an improved purification scheme that provided 20-fold increased enzyme yield over previous results. Structural characterization of electrophoretically pure TT-PME by MALDI-TOF MS determined molecular masses of approximately 47900 and 53000 Da for two principal glycoisoforms. De novo sequences generated from tryptic peptides by MALDI-TOF/TOF MS matched multiple anonymous Citrus EST cDNA accessions. The complete tt-pme cDNA (1710 base pair) was cloned from a fruit mRNA library using RT- and RLM-RACE PCR. Citrus TT-PME is a novel isoform that showed higher sequence identity with the multiply glycosylated kiwifruit PME than to previously described Citrus thermally labile PME isoforms.</div>
</front>
</TEI>
<affiliations><list><country><li>Inde</li>
<li>États-Unis</li>
</country>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Savary, Brett J" sort="Savary, Brett J" uniqKey="Savary B" first="Brett J." last="Savary">Brett J. Savary</name>
</noRegion>
<name sortKey="Cameron, Randall G" sort="Cameron, Randall G" uniqKey="Cameron R" first="Randall G." last="Cameron">Randall G. Cameron</name>
<name sortKey="Mccollum, T Gregory" sort="Mccollum, T Gregory" uniqKey="Mccollum T" first="T. Gregory" last="Mccollum">T. Gregory Mccollum</name>
<name sortKey="Nunez, Alberto" sort="Nunez, Alberto" uniqKey="Nunez A" first="Alberto" last="Nunez">Alberto Nunez</name>
<name sortKey="Savary, Brett J" sort="Savary, Brett J" uniqKey="Savary B" first="Brett J." last="Savary">Brett J. Savary</name>
<name sortKey="Vasu, Prasanna" sort="Vasu, Prasanna" uniqKey="Vasu P" first="Prasanna" last="Vasu">Prasanna Vasu</name>
</country>
<country name="Inde"><noRegion><name sortKey="Vasu, Prasanna" sort="Vasu, Prasanna" uniqKey="Vasu P" first="Prasanna" last="Vasu">Prasanna Vasu</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001113 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001113 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Bois
   |area=    OrangerV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     Pascal:14-0041424
   |texte=   Structural Characterization of the Thermally Tolerant Pectin Methylesterase Purified from Citrus sinensis Fruit and Its Gene Sequence
}}

This area was generated with Dilib version V0.6.25.
Data generation: Sat Dec 3 17:11:04 2016. Site generation: Wed Mar 6 18:18:32 2024

	Serveur d'exploration sur l'oranger
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur l'oranger

Structural Characterization of the Thermally Tolerant Pectin Methylesterase Purified from Citrus sinensis Fruit and Its Gene Sequence

Structural Characterization of the Thermally Tolerant Pectin Methylesterase Purified from Citrus sinensis Fruit and Its Gene Sequence

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri